Interleukin-22 drives the proliferation, migration and osteogenic differentiation of mesenchymal stem cells: a novel cytokine that could contribute to new bone formation …
AA El-Zayadi, EA Jones, SM Churchman… - …, 2017 - academic.oup.com
Rheumatology, 2017•academic.oup.com
Objectives. The SpAs are genetically and therapeutically linked to IL-23, which in turn
regulates IL-22, a cytokine that has been implicated in the regulation of new bone formation
in experimental models. We hypothesize that IL-22, a master regulator of stem cells in other
niches, might also regulate human mesenchymal stem cell (MSC) osteogenesis. Methods.
The effects of IL-22 on in vitro MSC proliferation, migration and osteogenic differentiation
were evaluated in the presence or absence of IFN-γ and TNF (to ascertain IL-22 activity in …
regulates IL-22, a cytokine that has been implicated in the regulation of new bone formation
in experimental models. We hypothesize that IL-22, a master regulator of stem cells in other
niches, might also regulate human mesenchymal stem cell (MSC) osteogenesis. Methods.
The effects of IL-22 on in vitro MSC proliferation, migration and osteogenic differentiation
were evaluated in the presence or absence of IFN-γ and TNF (to ascertain IL-22 activity in …
Objectives. The SpAs are genetically and therapeutically linked to IL-23, which in turn regulates IL-22, a cytokine that has been implicated in the regulation of new bone formation in experimental models. We hypothesize that IL-22, a master regulator of stem cells in other niches, might also regulate human mesenchymal stem cell (MSC) osteogenesis.
Methods. The effects of IL-22 on in vitro MSC proliferation, migration and osteogenic differentiation were evaluated in the presence or absence of IFN-γ and TNF (to ascertain IL-22 activity in pro-inflammatory environments). Colorimetric XTT assay, trans-well migration assays, quantitative real-time PCR (qRT-PCR) for MSC lineage markers and osteogenesis assays were used.
Results. Combined treatment of MSC with IL-22, IFN-γ and TNF resulted in increased MSC proliferation (P = 0.008) and migration (P = 0.04), an effect that was not seen in cells treated with IL-22 alone and untreated cells. Osteogenic and adipogenic, but not chondrogenic, transcription factors were upregulated by IL-22 alone (P < 0.05). MSC osteogenesis was enhanced following IL-22 exposure (P = 0.03, measured by calcium production). The combination of IFN-γ and TNF with or without IL-22 suppressed MSC osteogenesis (P = 0.03).
Conclusion. This work shows that IL-22 is involved in human MSC proliferation/migration in inflammatory environments, with MSC osteogenesis occurring only in the absence of IFN-γ/TNF. These effects of IL-22 on MSC function is a novel pathway for exploring pathological, post-inflammation osteogenesis in human SpA.
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