Rapid purification of native dynamin I and colorimetric GTPase assay
A Quan, PJ Robinson - Methods in enzymology, 2005 - Elsevier
Dynamin I is a large GTPase enzyme required in membrane constriction and fission during
multiple forms of endocytosis. The first method described here is for the rapid purification of
native dynamin from peripheral membrane extracts of sheep brain using ammonium sulfate
precipitation and affinity purification on recombinant SH3 domains. The method greatly
enriches for dynamin I at high purity and allows for large‐scale biochemical and functional
studies. The second method is a nonradioactive, high‐throughput colorimetric GTPase …
multiple forms of endocytosis. The first method described here is for the rapid purification of
native dynamin from peripheral membrane extracts of sheep brain using ammonium sulfate
precipitation and affinity purification on recombinant SH3 domains. The method greatly
enriches for dynamin I at high purity and allows for large‐scale biochemical and functional
studies. The second method is a nonradioactive, high‐throughput colorimetric GTPase …