B‐cell commitment, development and selection

RR Hardy, YS Li, D Allman, M Asano… - Immunological …, 2000 - Wiley Online Library
RR Hardy, YS Li, D Allman, M Asano, M Gui, K Hayakawa
Immunological reviews, 2000Wiley Online Library
Here we review three areas in B‐cell development in the mouse, with a focus on relevance
to B‐1/CD5+ B cells. Multiparameter flow cytometry has allowed the dissection of
intermediate stages of developing B cells, both in fetal liver and bone marrow. In the first
area, we present recent work that has delineated a fraction of pre‐pro‐B cells, committed to
the B lineage, but lacking any immunoglobulin rearrangements. Next, the role of the pre‐B‐
cell receptor in B‐cell repertoire selection has become clear in the past few years, but we …
Summary
Here we review three areas in B‐cell development in the mouse, with a focus on relevance to B‐1/CD5+ B cells. Multiparameter flow cytometry has allowed the dissection of intermediate stages of developing B cells, both in fetal liver and bone marrow. In the first area, we present recent work that has delineated a fraction of pre‐pro‐B cells, committed to the B lineage, but lacking any immunoglobulin rearrangements. Next, the role of the pre‐B‐cell receptor in B‐cell repertoire selection has become clear in the past few years, but we present work suggesting that the action of this process during fetal life is different, resulting in selection of a very distinct repertoire compared with adult. Finally, we describe a new VH3609 antithymocyte Ig transgenic mouse model system that has provided the first definitive evidence for the role of self‐antigen in development and maintenance of natural autoreactive B cells.
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