A general and fast method to generate multiple site directed mutations.
I Mikaelian, A Sergeant - Nucleic acids research, 1992 - ncbi.nlm.nih.gov
I Mikaelian, A Sergeant
Nucleic acids research, 1992•ncbi.nlm.nih.govMany methods have been described for site directed mutagenesis. One ofthe most powerful
ones is the overlap extension method described by Higuchi et al.(1, 2), but this method
requires two new primers for each mutagenesis and for this reason is not adapted for an
extensive mutagenesis in the same DNA sequence. We have developed a rapid and
efficient method derived from the overlap extension method which allows one to generate
multiple site directed mutations (deletion, insertion or substitution) in a given DNA fragment …
ones is the overlap extension method described by Higuchi et al.(1, 2), but this method
requires two new primers for each mutagenesis and for this reason is not adapted for an
extensive mutagenesis in the same DNA sequence. We have developed a rapid and
efficient method derived from the overlap extension method which allows one to generate
multiple site directed mutations (deletion, insertion or substitution) in a given DNA fragment …
Many methods have been described for site directed mutagenesis. One ofthe most powerful ones is the overlap extension method described by Higuchi et al.(1, 2), but this method requires two new primers for each mutagenesis and for this reason is not adapted for an extensive mutagenesis in the same DNA sequence.
We have developed a rapid and efficient method derived from the overlap extension method which allows one to generate multiple site directed mutations (deletion, insertion or substitution) in a given DNA fragment. It requires three universal primers chosen in the vector and only one specific primer for each mutation.
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