[HTML][HTML] P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-α and prostaglandins
M Domercq, L Brambilla, E Pilati, J Marchaland… - Journal of Biological …, 2006 - ASBMB
ATP, released by both neurons and glia, is an important mediator of brain intercellular
communication. We find that selective activation of purinergic P2Y1 receptors (P2Y1R) in
cultured astrocytes triggers glutamate release. By total internal fluorescence reflection
imaging of fluorescence-labeled glutamatergic vesicles, we document that such release
occurs by regulated exocytosis. The stimulus-secretion coupling mechanism involves Ca 2+
release from internal stores and is controlled by additional transductive events mediated by …
communication. We find that selective activation of purinergic P2Y1 receptors (P2Y1R) in
cultured astrocytes triggers glutamate release. By total internal fluorescence reflection
imaging of fluorescence-labeled glutamatergic vesicles, we document that such release
occurs by regulated exocytosis. The stimulus-secretion coupling mechanism involves Ca 2+
release from internal stores and is controlled by additional transductive events mediated by …
