[HTML][HTML] A distinct class of dominant negative Ras mutants: cytosolic GTP-bound Ras effector domain mutants that inhibit Ras signaling and transformation and …
JJ Fiordalisi, SP Holly, RL Johnson, LV Parise… - Journal of Biological …, 2002 - ASBMB
Cytosolic GTP-bound Ras has been shown to act as a dominant negative (DN) inhibitor of
Ras by sequestering Raf in non-productive cytosolic complexes. Nevertheless, this distinct
class of DN mutants has been neither well characterized nor extensively used to analyze
Ras signaling. In contrast, DN Ras17N, which functions by blocking Ras guanine nucleotide
exchange factors, has been well characterized and is widely used. Cytosolic GTP-bound
Ras mutants could be used to inhibit particular Ras effectors by introducing additional …
Ras by sequestering Raf in non-productive cytosolic complexes. Nevertheless, this distinct
class of DN mutants has been neither well characterized nor extensively used to analyze
Ras signaling. In contrast, DN Ras17N, which functions by blocking Ras guanine nucleotide
exchange factors, has been well characterized and is widely used. Cytosolic GTP-bound
Ras mutants could be used to inhibit particular Ras effectors by introducing additional …
