[HTML][HTML] SNAREs contribute to the specificity of membrane fusion
SJ Scales, YA Chen, BY Yoo, SM Patel, YC Doung… - Neuron, 2000 - cell.com
SJ Scales, YA Chen, BY Yoo, SM Patel, YC Doung, RH Scheller
Neuron, 2000•cell.comIntracellular membrane fusion is mediated by the formation of a four-helix bundle comprised
of SNARE proteins. Every cell expresses a large number of SNARE proteins that are
localized to particular membrane compartments, suggesting that the fidelity of vesicle
trafficking might in part be determined by specific SNARE pairing. However, the promiscuity
of SNARE pairing in vitro suggests that the information for membrane compartment
organization is not encoded in the inherent ability of SNAREs to form complexes. Here, we …
of SNARE proteins. Every cell expresses a large number of SNARE proteins that are
localized to particular membrane compartments, suggesting that the fidelity of vesicle
trafficking might in part be determined by specific SNARE pairing. However, the promiscuity
of SNARE pairing in vitro suggests that the information for membrane compartment
organization is not encoded in the inherent ability of SNAREs to form complexes. Here, we …
Abstract
Intracellular membrane fusion is mediated by the formation of a four-helix bundle comprised of SNARE proteins. Every cell expresses a large number of SNARE proteins that are localized to particular membrane compartments, suggesting that the fidelity of vesicle trafficking might in part be determined by specific SNARE pairing. However, the promiscuity of SNARE pairing in vitro suggests that the information for membrane compartment organization is not encoded in the inherent ability of SNAREs to form complexes. Here, we show that exocytosis of norepinephrine from PC12 cells is only inhibited or rescued by specific SNAREs. The data suggest that SNARE pairing does underlie vesicle trafficking fidelity, and that specific SNARE interactions with other proteins may facilitate the correct pairing.
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