Covalent attachment of human complement C3 to IgG. Identification of the amino acid residue involved in ester linkage formation.
A Sahu, MK Pangburn - Journal of Biological Chemistry, 1994 - ASBMB
C3 (native complement component 3) plays a central role in the activation of complement
and in the transport and processing of immune complexes. Proteolytic activation of C3
exposes a highly reactive thioester bond which preferentially reacts with the hydroxyl groups
of acceptor molecules on activators such as immune complexes or carbohydrates on
microorganisms. Recently, a C3 attachment site has been localized on the CH1 domain of
IgG1 between Val134 and Lys156. We have synthesized a series of peptide analogs of this …
and in the transport and processing of immune complexes. Proteolytic activation of C3
exposes a highly reactive thioester bond which preferentially reacts with the hydroxyl groups
of acceptor molecules on activators such as immune complexes or carbohydrates on
microorganisms. Recently, a C3 attachment site has been localized on the CH1 domain of
IgG1 between Val134 and Lys156. We have synthesized a series of peptide analogs of this …