Characterization of a unique factor-independent variant derived from human factor-dependent TF-1 cells: a transformed event
X Hu, LC Moscinski, BJ Hill, Q Chen, J Wu, AB Fisher… - Leukemia research, 1998 - Elsevier
A factor-independent variant (TF-1a) has been isolated from the factor-dependent TF-1 cell
line. The subline has been grown continuously in culture for> 1.5 years without added
cytokines. The cells retain the ability to respond to multicytokines, with a different response
pattern from its parental cell line. The TF-1 cells appeared singly in liquid culture. In contrast,
TF-la cells formed aggregates which increased markedly in size and in number upon TGFβ1
treatment and showed a diminished TGFβ-mediated growth inhibition. TF-la, but not TF-1 …
line. The subline has been grown continuously in culture for> 1.5 years without added
cytokines. The cells retain the ability to respond to multicytokines, with a different response
pattern from its parental cell line. The TF-1 cells appeared singly in liquid culture. In contrast,
TF-la cells formed aggregates which increased markedly in size and in number upon TGFβ1
treatment and showed a diminished TGFβ-mediated growth inhibition. TF-la, but not TF-1 …