Propagation of human spermatogonial stem cells in vitro
H Sadri-Ardekani, SC Mizrak, SKM van Daalen… - Jama, 2009 - jamanetwork.com
Context Young boys treated with high-dose chemotherapy are often confronted with infertility
once they reach adulthood. Cryopreserving testicular tissue before chemotherapy and
autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for
restoration of fertility. Objective To establish in vitro propagation of human spermatogonial
stem cells from small testicular biopsies to obtain an adequate number of cells for successful
transplantation. Design, Setting, and Participants Study performed from April 2007 to July …
once they reach adulthood. Cryopreserving testicular tissue before chemotherapy and
autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for
restoration of fertility. Objective To establish in vitro propagation of human spermatogonial
stem cells from small testicular biopsies to obtain an adequate number of cells for successful
transplantation. Design, Setting, and Participants Study performed from April 2007 to July …