[HTML][HTML] Expression of a dominant negative heat shock factor-1 construct inhibits aneuploidy in prostate carcinoma cells
Y Wang, JR Theriault, H He, J Gong… - Journal of Biological …, 2004 - ASBMB
Recent studies have implicated heat shock proteins (HSP) and heat shock transcription
factor 1 (HSF1) in tumor progression. We have examined the role of HSF1 in the malignant
phenotype of PC-3 prostate carcinoma cells. We have developed a dominant negative
construct of HSF1 that antagonizes transcription from HSP promoters and results in the
depletion of intracellular HSP 70. Our studies indicate that expression of DN-HSF1
dramatically alters the DNA content of PC-3 cells (derived from p53 null prostatic carcinoma) …
factor 1 (HSF1) in tumor progression. We have examined the role of HSF1 in the malignant
phenotype of PC-3 prostate carcinoma cells. We have developed a dominant negative
construct of HSF1 that antagonizes transcription from HSP promoters and results in the
depletion of intracellular HSP 70. Our studies indicate that expression of DN-HSF1
dramatically alters the DNA content of PC-3 cells (derived from p53 null prostatic carcinoma) …