Characterization of non-toxic mutant toxins of Vero toxin 1 that were constructed by replacing amino acids in the A subunit
M Ohmura, S Yamasaki, H Kurazono, K Kashiwagi… - Microbial …, 1993 - Elsevier
Three mutant toxins of Vero toxin 1 (VT1) produced by Escherichia coli strains carrying
mutant VT1 genes that were constructed by oligonucleotide-directed site-specific
mutagenesis were purified to homogeneity. They are E167Q (glutamic acid at position 167
from the N-terminus of the A subunit was replaced by glutamine), R170L (arginine at
position 170 from the N-terminus of the A subunit was replaced by leucine) and E167Q-
R170L (glutamic acid at position 167 and arginine at position 170 were replaced by …
mutant VT1 genes that were constructed by oligonucleotide-directed site-specific
mutagenesis were purified to homogeneity. They are E167Q (glutamic acid at position 167
from the N-terminus of the A subunit was replaced by glutamine), R170L (arginine at
position 170 from the N-terminus of the A subunit was replaced by leucine) and E167Q-
R170L (glutamic acid at position 167 and arginine at position 170 were replaced by …