Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli
MP Jackson, JW Newland, RK Holmes… - Microbial pathogenesis, 1987 - Elsevier
A nucleotide sequence analysis was performed on the Shiga-like toxin I genes previously
cloned from the Escherichia coli bacteriophage 933J. Two structural genes designated slt-IA
and slt-IB were found to be oriented on a single transcriptional unit with slt-IA preceding slt-I
B. A 12 base pair gap separated the in-phase open reading frames of slt-IA and slt-I B.
Putative ribosome binding sites were identified 5′ to both the slt-IA and slt-IB genes.
Translation of the SLT-I nucleotide sequence revealed that both the A and B subunits were …
cloned from the Escherichia coli bacteriophage 933J. Two structural genes designated slt-IA
and slt-IB were found to be oriented on a single transcriptional unit with slt-IA preceding slt-I
B. A 12 base pair gap separated the in-phase open reading frames of slt-IA and slt-I B.
Putative ribosome binding sites were identified 5′ to both the slt-IA and slt-IB genes.
Translation of the SLT-I nucleotide sequence revealed that both the A and B subunits were …