Transepithelial chloride and fluid secretion by many types of epithelia involves activation of
a conductive K+ pathway that serves to support the electrochemical driving force for Cl−
secretion. This study sought to determine if such a pathway is involved in Cl− and fluid
secretion by the cystic epithelia in autosomal dominant polycystic kidney disease (ADPKD).
Primary cultures of cells derived from the cysts of patients with ADPKD were used. Confluent
monolayers of these cells, mounted in Ussing chambers, were stimulated to secrete Cl− by …