[HTML][HTML] Arachidonic acid release from mammalian cells transfected with human groups IIA and X secreted phospholipase A2 occurs predominantly during the …
CM Mounier, F Ghomashchi, MR Lindsay… - Journal of Biological …, 2004 - ASBMB
Stable expression of human groups IIA and X secreted phospholipases A 2 (hGIIA and hGX)
in CHO-K1 and HEK293 cells leads to serum-and interleukin-1β-promoted arachidonate
release. Using mutant CHO-K1 cell lines, it is shown that this arachidonate release does not
require heparan sulfate proteoglycan-or glycosylphosphatidylinositol-anchored proteins. It is
shown that the potent secreted phospholipase A 2 inhibitor Me-Indoxam is cell-
impermeable. By use of Me-Indoxam and the cell-impermeable, secreted phospholipase A 2 …
in CHO-K1 and HEK293 cells leads to serum-and interleukin-1β-promoted arachidonate
release. Using mutant CHO-K1 cell lines, it is shown that this arachidonate release does not
require heparan sulfate proteoglycan-or glycosylphosphatidylinositol-anchored proteins. It is
shown that the potent secreted phospholipase A 2 inhibitor Me-Indoxam is cell-
impermeable. By use of Me-Indoxam and the cell-impermeable, secreted phospholipase A 2 …
