CpNifS, a cysteine desulfurase required to supply sulfur for ironsulfur cluster biogenesis in
Arabidopsis thaliana chloroplasts, belongs to a class of NifS-like enzymes with low
endogenous cysteine desulfurase activity. Its bacterial homologue SufS is stimulated by
SufE. Here we characterize the Arabidopsis chloroplast protein CpSufE, which has an N-
terminal SufE-like domain and a C-terminal BolA-like domain unique to higher plants.
CpSufE is targeted to the chloroplast stroma, indicated by green fluorescent protein …