Inhibition of the c-Jun N-terminal kinase/AP-1 and NF-κB pathways by PICOT, a novel protein kinase C-interacting protein with a thioredoxin homology domain
S Witte, M Villalba, K Bi, Y Liu, N Isakov… - Journal of Biological …, 2000 - ASBMB
Protein kinase C-θ (PKCθ) is a Ca 2+-independent PKC isoform that is selectively
expressed in T lymphocytes (and muscle), and is thought to play an important role in T cell
receptor-induced activation. To gain a better understanding of the function and regulation of
PKCθ, we have employed the yeast two-hybrid system to identify PKCθ-interacting proteins.
We report the isolation and characterization of a cDNA encoding a novel 335-amino acid
(37.5-kDa) PKCθ-interacting protein termed PICOT (for PKC-interactingcousin of …
expressed in T lymphocytes (and muscle), and is thought to play an important role in T cell
receptor-induced activation. To gain a better understanding of the function and regulation of
PKCθ, we have employed the yeast two-hybrid system to identify PKCθ-interacting proteins.
We report the isolation and characterization of a cDNA encoding a novel 335-amino acid
(37.5-kDa) PKCθ-interacting protein termed PICOT (for PKC-interactingcousin of …
