[HTML][HTML] Control of retinoic acid receptor heterodimerization by ligand-induced structural transitions: A novel mechanism of action for retinoid antagonists
C Depoix, MH Delmotte, P Formstecher… - Journal of Biological …, 2001 - ASBMB
Heterodimerization of retinoic acid receptors (RARs) with 9-cis-retinoic receptors (RXRs) is a
prerequisite for binding of RXR· RAR dimers to DNA and for retinoic acid-induced gene
regulation. Whether retinoids control RXR/RAR solution interaction remains a debated
question, and we have usedin vitro and in vivo protein interaction assays to investigate the
role of ligand in modulating RXR/RAR interaction in the absence of DNA. Two-hybrid assay
in mammalian cells demonstrated that only RAR agonists were able to increase significantly …
prerequisite for binding of RXR· RAR dimers to DNA and for retinoic acid-induced gene
regulation. Whether retinoids control RXR/RAR solution interaction remains a debated
question, and we have usedin vitro and in vivo protein interaction assays to investigate the
role of ligand in modulating RXR/RAR interaction in the absence of DNA. Two-hybrid assay
in mammalian cells demonstrated that only RAR agonists were able to increase significantly …
