A mouse model of sensorimotor controlled cortical impact: characterization using longitudinal magnetic resonance imaging, behavioral assessments and histology

G Onyszchuk, B Al-Hafez, YY He, M Bilgen… - Journal of neuroscience …, 2007 - Elsevier
G Onyszchuk, B Al-Hafez, YY He, M Bilgen, NEJ Berman, WM Brooks
Journal of neuroscience methods, 2007Elsevier
The present study establishes a new mouse model for traumatic brain injury (TBI), using an
electromechanically driven linear motor impactor device to deliver a lateral controlled
cortical impact (CCI) injury to the sensorimotor cortex. Lesion cavity size was measured, and
inter-animal consistency demonstrated, at 14 days post injury. Qualitative information
regarding damage progression over time was obtained by scanning with high field magnetic
resonance imaging (MRI) at five time points following injury. Functional impairment and …
The present study establishes a new mouse model for traumatic brain injury (TBI), using an electromechanically driven linear motor impactor device to deliver a lateral controlled cortical impact (CCI) injury to the sensorimotor cortex. Lesion cavity size was measured, and inter-animal consistency demonstrated, at 14 days post injury. Qualitative information regarding damage progression over time was obtained by scanning with high field magnetic resonance imaging (MRI) at five time points following injury. Functional impairment and recovery were measured with the Rotarod, gridwalk and cylinder tests, and lesion cavity volume was measured post mortem with thionin-stained tissue sections. The study establishes the reliability of a linear-motor based device for producing repeatable damage in a CCI model, demonstrates the power of longitudinal MRI in studying damage evolution, and confirms that a simple battery of functional tests record sensorimotor impairment and recovery.
Elsevier