Argininosuccinate synthase expression is required to maintain nitric oxide production and cell viability in aortic endothelial cells
BL Goodwin, LP Solomonson, DC Eichler - Journal of Biological Chemistry, 2004 - ASBMB
Although cellular levels of arginine greatly exceed the apparent K m for endothelial nitric-
oxide synthase, current evidence suggests that the bulk of this arginine may not be available
for nitric oxide (NO) production. We propose that arginine regeneration, that is the recycling
of citrulline back to arginine, defines the essential source of arginine for NO production. To
support this proposal, RNA interference analysis was used to selectively reduce the
expression of argininosuccinate synthase (AS), because the only known metabolic role for …
oxide synthase, current evidence suggests that the bulk of this arginine may not be available
for nitric oxide (NO) production. We propose that arginine regeneration, that is the recycling
of citrulline back to arginine, defines the essential source of arginine for NO production. To
support this proposal, RNA interference analysis was used to selectively reduce the
expression of argininosuccinate synthase (AS), because the only known metabolic role for …
