Structural Basis for Phosphatidylinositol Phosphate Kinase Type Iγ Binding to Talin at Focal Adhesions*[boxs]
JM de Pereda, KL Wegener, E Santelli, N Bate… - Journal of Biological …, 2005 - ASBMB
The cytoskeletal protein talin binds to a short C-terminal sequence in phosphatidylinositol
phosphate kinase type Iγ (PIPKIγ), activating the enzyme and promoting the local production
of phosphatidylinositol 4, 5 bisphosphate, which regulates focal adhesion dynamics as well
as clathrin-mediated endocytosis in neuronal cells. Here we show by crystallographic, NMR,
and calorimetric analysis that the phosphotyrosine binding (PTB)-like domain of talin
engages the PIPKIγ C terminus in a mode very similar to that of integrin binding. However …
phosphate kinase type Iγ (PIPKIγ), activating the enzyme and promoting the local production
of phosphatidylinositol 4, 5 bisphosphate, which regulates focal adhesion dynamics as well
as clathrin-mediated endocytosis in neuronal cells. Here we show by crystallographic, NMR,
and calorimetric analysis that the phosphotyrosine binding (PTB)-like domain of talin
engages the PIPKIγ C terminus in a mode very similar to that of integrin binding. However …
