The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin β1
The sex-determining factor SRY is a DNA-binding protein that diverts primordial gonads
from the ovarian pathway toward male differentiation to form testes. It gains access to the
nucleus through two distinct nuclear localization signals (NLSs) that flank the high mobility
group (HMG) DNA-binding domain, but the mechanisms through which these NLSs operate
have not been studied. In this study, we reconstitute the nuclear import of SRY in vitro,
demonstrating a lack of requirement for exogenous factors for nuclear accumulation and a …
from the ovarian pathway toward male differentiation to form testes. It gains access to the
nucleus through two distinct nuclear localization signals (NLSs) that flank the high mobility
group (HMG) DNA-binding domain, but the mechanisms through which these NLSs operate
have not been studied. In this study, we reconstitute the nuclear import of SRY in vitro,
demonstrating a lack of requirement for exogenous factors for nuclear accumulation and a …
