[HTML][HTML] DGAT1 overexpression in muscle by in vivo DNA electroporation increases intramyocellular lipid content
BD Roorda, MKC Hesselink, G Schaart… - Journal of lipid …, 2005 - ASBMB
In adipose tissue, the microsomal enzyme 1, 2-acyl CoA: diacylglyceroltransferase-1
(DGAT1) plays an important role in triglyceride storage. Because DGAT1 is expressed in
skeletal muscle as well, we aimed to directly test the effect of DGAT1 on muscular
triglyceride storage by overexpressing DGAT1 using in vivo DNA electroporation. A
pcDNA3. 1-DGAT1 construct in saline was injected in the left tibialis anterior muscle of rats,
followed by the application of eight transcutaneous pulses, using the contralateral leg as …
(DGAT1) plays an important role in triglyceride storage. Because DGAT1 is expressed in
skeletal muscle as well, we aimed to directly test the effect of DGAT1 on muscular
triglyceride storage by overexpressing DGAT1 using in vivo DNA electroporation. A
pcDNA3. 1-DGAT1 construct in saline was injected in the left tibialis anterior muscle of rats,
followed by the application of eight transcutaneous pulses, using the contralateral leg as …
