Renal clearance of ferrocyanide in the dog
RW Berliner, TJ Kennedy JR… - American Journal of …, 1950 - journals.physiology.org
RW Berliner, TJ Kennedy JR, JG Hilton
American Journal of Physiology-Legacy Content, 1950•journals.physiology.orgMATERIALS AND METHODS Studies were performed on trained, normal female dogs
weighing 12 to 20 kg. Creatinine to yield plasma concentrations of about 20 mg. per cent
and ferrocyanide were administered by continuous intravenous infusion. Urines were
collected through an indwelling catheter and each clearance period terminated by washing
the bladder with distilled water. Heparinized arterial blood samples were obtained at the
midpoint of each clearance period. Ferrocyanide in blood and urine was determined by a …
weighing 12 to 20 kg. Creatinine to yield plasma concentrations of about 20 mg. per cent
and ferrocyanide were administered by continuous intravenous infusion. Urines were
collected through an indwelling catheter and each clearance period terminated by washing
the bladder with distilled water. Heparinized arterial blood samples were obtained at the
midpoint of each clearance period. Ferrocyanide in blood and urine was determined by a …
MATERIALS AND METHODS
Studies were performed on trained, normal female dogs weighing 12 to 20 kg. Creatinine to yield plasma concentrations of about 20 mg. per cent and ferrocyanide were administered by continuous intravenous infusion. Urines were collected through an indwelling catheter and each clearance period terminated by washing the bladder with distilled water. Heparinized arterial blood samples were obtained at the midpoint of each clearance period.
Ferrocyanide in blood and urine was determined by a method which utilizes the spectral absorption peak of ferricyanide at a wave length of 420 p as follows: Plasma. To 2 ml. of plasma add IO ml. of distilled water and 8 ml. of IO per cent vacuum-distilled2 trichloroacetic acid. Filter. To duplicate 5 ml. aliquots of filtrate
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