Use of class II tetramers for identification of CD4+ T cells
WW Kwok, NA Ptacek, AW Liu, JH Buckner - Journal of immunological …, 2002 - Elsevier
Multivalent MHC class II molecules containing peptide antigens are useful tools for the
detection of antigen specific human CD4+ T cells. Tetramers produced by exogenous
peptide loading onto empty class II molecules are comparable to tetramers with peptide
tethered to the class II chain covalently, but have many practical advantages. Conditions for
optimal peptide loading to generate tetramers are discussed and optimal conditions of using
tetramers for staining T cells are examined. As the frequency of antigen specific CD4+ T …
detection of antigen specific human CD4+ T cells. Tetramers produced by exogenous
peptide loading onto empty class II molecules are comparable to tetramers with peptide
tethered to the class II chain covalently, but have many practical advantages. Conditions for
optimal peptide loading to generate tetramers are discussed and optimal conditions of using
tetramers for staining T cells are examined. As the frequency of antigen specific CD4+ T …
