Molecular cloning of the CA125 ovarian cancer antigen: identification as a new mucin, MUC16
BWT Yin, KO Lloyd - Journal of Biological Chemistry, 2001 - ASBMB
CA125 is an ovarian cancer antigen that is the basis for a widely used serum assay for the
monitoring of patients with ovarian cancer; however, detailed information on its biochemical
and molecular nature is lacking. We now report the isolation of a long, but partial, cDNA that
corresponds to the CA125 antigen. A rabbit polyclonal antibody produced to purified CA125
antigen was used to screen a λZAP cDNA library from OVCAR-3 cells inEscherichia coli.
The longest insert from the 54 positive isolated clones had a 5797-base pair sequence …
monitoring of patients with ovarian cancer; however, detailed information on its biochemical
and molecular nature is lacking. We now report the isolation of a long, but partial, cDNA that
corresponds to the CA125 antigen. A rabbit polyclonal antibody produced to purified CA125
antigen was used to screen a λZAP cDNA library from OVCAR-3 cells inEscherichia coli.
The longest insert from the 54 positive isolated clones had a 5797-base pair sequence …
